Gaming centers also known as "internet cafes" are likely to become illegal in Florida by nightfall.
Gov. Rick Scott is expected to sign a bill Wednesday that would ban up to 1,000 storefront operations across the state.
The new law takes effect immediately after Scott signs the bill.
However, state officials say they will leave enforcement of the new law to local law-enforcement officials.
Florida legislators voted overwhelmingly last week to approve the ban. It was a quick response to a scandal that led to dozens of arrests and the lieutenant governor's resignation last month.
The storefront operations targeted by the bill currently allow customers to play sweepstakes games that simulate slot machines. But critics contend the new law will also lead to the closures of senior arcades.